Hydrochloric Acid (1M)

Author

Affiliations

Pranav Kumar Mishra

Dept of Surgery, Rush University

Dept of Orthopedic Surgery, Rush University

Overview

Uses

Titrating pH, destruction of RNAse on glass slides

Components

1. \(diH_2O\)
2. 37% \(HCl\) (Sigma-Aldrich)
3. Sterile container

Protocol: Preparation of 500mL 1M (1N) HCl

1. In a vented hood, add \(100\ mL\) of \(diH_2O\) to a beaker with a stir bar
2. Add \(37 g\ HCl\) to the beaker and mix thoroughly to make \(37\%\ HCl\) (\(12.18\ M\ HCl\))
3. Add \(41.05\ mL\) of \(37\%\ HCl\) to the beaker
4. Dilute to a total of \(500\ mL\) with \(diH_2O\)
5. Transfer into a glass container that seals adequately

Calculation

\[ \frac{37\ g\ HCl}{100\ mL} x \frac{1.2\ g}{mL} x \frac{1\ mol\ HCl}{36.46\ g} x \frac{1000\ mL}{1 \ L} = 12.18\ M\ HCl \]

\[ (12.18\ M\ HCl)(500\ mL) = (1\ M\ HCl)(41.05\ mL) \]

Storage

• Store in an appropriate corrosive reagent storage